Phospho-VASP (Ser239) Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3466R |
| Product Name | Phospho-VASP (Ser239) Polyclonal Antibody |
| Applications |
WB
ELISA
|
| Specificity | This phosphorylation site is homologous to that of Ser235 in Mouse and Ser236 in Rat. |
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human VASP around the phosphorylation site of Ser239 |
| Modification Site | Ser239 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 7408 |
| Swiss Prot | P50552 |
| Subcellular location | Cytoplasm |
| Synonyms | Vasodilator-stimulated phosphoprotein; VASP |
| Background | Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
Frequently asked questions
Which applications is Phospho-VASP (Ser239) Polyclonal Antibody (bs-3466R) validated for, and at what dilution?
bs-3466R is validated for WB (1:300-5000); ELISA (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-VASP (Ser239) Polyclonal Antibody (bs-3466R) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow.
How should bs-3466R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-VASP (Ser239) Polyclonal Antibody (bs-3466R)?
KLH conjugated synthetic phosphopeptide derived from human VASP around the phosphorylation site of Ser239