phospho-Tau (Ser422) Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3489R-RBITC-100uL |
| Product Name | phospho-Tau (Ser422) Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylation site is homologous to that of Ser714 in Mouse and Ser733 in Rat. |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Rabbit |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Tau protein around the phosphorylation site of Ser739 [VD(p-S)PQ] |
| Modification Site | Ser739 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4137 |
| Swiss Prot | P10636 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103; Microtubule-associated protein tau; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau; MAPT |
| Background | Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-Tau (Ser422) Polyclonal Antibody, RBITC Conjugated (bs-3489R-RBITC) validated for, and at what dilution?
bs-3489R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Tau (Ser422) Polyclonal Antibody, RBITC Conjugated (bs-3489R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Rabbit.
How should bs-3489R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Tau (Ser422) Polyclonal Antibody, RBITC Conjugated (bs-3489R-RBITC)?
KLH conjugated synthetic phosphopeptide derived from human Tau protein around the phosphorylation site of Ser739 [VD(p-S)PQ]
What are the excitation and emission wavelengths of the RBITC conjugate (bs-3489R-RBITC)?
RBITC on bs-3489R-RBITC: excitation / emission 540nm/625nm.