Phospho-NCF4 (Thr154) Polyclonal Antibody, Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-3497R-Cy5 |
| Product Name | Phospho-NCF4 (Thr154) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Pig, Rabbit, Guinea Pig |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human p40phox around the phosphorylation site of Thr154 |
| Modification Site | Thr154 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4689 |
| Subcellular location | Cytoplasm |
| Synonyms | MGC3810; NCF 4; NCF; NCF-4; Ncf4; NCF4_HUMAN; Neutrophil cytosol factor 4; Neutrophil cytosolic factor 4; Neutrophil NADPH oxidase factor 4; p40-phox; p40phox; SH3 and PX domain-containing protein 4. |
| Background | NCF4 is a cytosolic oxidase associated protein. It is a component of the nicotinamide adenine dinucleotide phosphate oxidase, which mediates down regulation of NADPH oxidase through interactions with its SH3 domain. NCF4 is associated with p67 phox but is absent in patients with chronic granulomatous disease who lack p67 phox. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-NCF4 (Thr154) Polyclonal Antibody, Cy5 Conjugated (bs-3497R-Cy5) validated for, and at what dilution?
bs-3497R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-3497R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-NCF4 (Thr154) Polyclonal Antibody, Cy5 Conjugated (bs-3497R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human p40phox around the phosphorylation site of Thr154
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-3497R-Cy5)?
Cy5 on bs-3497R-Cy5: excitation / emission 625,650nm/670nm.