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Synapsin 1 Polyclonal Antibody, AbBy Fluor-488 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Pig
Overview
Catalog # bs-3501R-BF488
Product Name Synapsin 1 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity This antibody will detect both isoforms of Synapsin 1.
Reactivity Human, Rat
Predicted Reactivity Mouse, Pig
Specifications
Conjugation AbBy Fluor-488
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Synapsin I
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6853
Swiss Prot P17600
Subcellular location Cytoplasm, Cell membrane
Synonyms SYNI; SYN1a; SYN1b; Synapsin-1; Brain protein 4.1; Synapsin I; SYN1
Background Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Synapsin 1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-3501R-BF488) validated for, and at what dilution?

bs-3501R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Synapsin 1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-3501R-BF488) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Pig.

How should bs-3501R-BF488 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Synapsin 1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-3501R-BF488)?

KLH conjugated synthetic peptide derived from human Synapsin I

What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-3501R-BF488)?

AbBy Fluor-488 on bs-3501R-BF488: excitation / emission 499nm/519nm.