Synapsin 1 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3501R-RBITC-100uL |
| Product Name | Synapsin 1 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This antibody will detect both isoforms of Synapsin 1. |
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Pig |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Synapsin I |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6853 |
| Swiss Prot | P17600 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | SYNI; SYN1a; SYN1b; Synapsin-1; Brain protein 4.1; Synapsin I; SYN1 |
| Background | Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Synapsin 1 Polyclonal Antibody, RBITC Conjugated (bs-3501R-RBITC) validated for, and at what dilution?
bs-3501R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Synapsin 1 Polyclonal Antibody, RBITC Conjugated (bs-3501R-RBITC) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Pig.
How should bs-3501R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Synapsin 1 Polyclonal Antibody, RBITC Conjugated (bs-3501R-RBITC)?
KLH conjugated synthetic peptide derived from human Synapsin I
What are the excitation and emission wavelengths of the RBITC conjugate (bs-3501R-RBITC)?
RBITC on bs-3501R-RBITC: excitation / emission 540nm/625nm.