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Myt1 Polyclonal Antibody, Cy5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Horse
Overview
Catalog # bs-3505R-Cy5-100uL
Product Name Myt1 Polyclonal Antibody, Cy5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Horse
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Myt1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4661
Subcellular location Nucleus
Synonyms C20orf36; MTF1; Myelin transcription factor 1; Myelin transcription factor I; MyT1; MYT1_HUMAN; MyTI; PLPB1; Proteolipid protein-binding protein.
Background Myt1 is a zinc finger protein that is known to interact with the co-repressor Sin3B and also HDAC1 and HDAC2. The Myt1 family, including Myt1 and Myt1L, exemplifies a class of neural sequence specific transcription factors that actively recruit HDACs to selected genes during CNS development.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Myt1 Polyclonal Antibody, Cy5 Conjugated (bs-3505R-Cy5) validated for, and at what dilution?

bs-3505R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Myt1 Polyclonal Antibody, Cy5 Conjugated (bs-3505R-Cy5) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Horse.

How should bs-3505R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Myt1 Polyclonal Antibody, Cy5 Conjugated (bs-3505R-Cy5)?

KLH conjugated synthetic peptide derived from human Myt1

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-3505R-Cy5)?

Cy5 on bs-3505R-Cy5: excitation / emission 625,650nm/670nm.