CTDSPL Polyclonal Antibody, PE-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3509R-PE-Cy5.5-100uL |
| Product Name | CTDSPL Polyclonal Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Chicken, Rabbit |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CTDSPL |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10217 |
| Swiss Prot | O15194 |
| Subcellular location | Nucleus |
| Synonyms | PSR1; SCP3; HYA22; RBSP3; C3orf8; CTD small phosphatase-like protein; CTDSP-like; Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3; NIF-like protein; Nuclear LIM interactor-interacting factor 1; NLI-interacting factor 1; Protein YA22; Small C-terminal domain phosphatase 3; Small CTD phosphatase 3; CTDSPL; NIF1; NIFL; YA22 |
| Background | Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells (By similarity). Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is CTDSPL Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-3509R-PE-CY5-5) validated for, and at what dilution?
BS-3509R-PE-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CTDSPL Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-3509R-PE-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Chicken, Rabbit.
How should BS-3509R-PE-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CTDSPL Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-3509R-PE-CY5-5)?
KLH conjugated synthetic peptide derived from human CTDSPL
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BS-3509R-PE-CY5-5)?
PE-Cy5.5 on BS-3509R-PE-CY5-5: excitation / emission 488nm/694nm.