WBSCR14 Polyclonal Antibody, APC-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3516R-APC-Cy5.5-100uL |
| Product Name | WBSCR14 Polyclonal Antibody, APC-Cy5.5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | APC-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human WBSCR14/ChREBP |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 51085 |
| Subcellular location | Nucleus |
| Synonyms | ChREBP; bHLHd14; Carbohydrate responsive element binding protein; MIO; MLX interacting protein like; Mlx interactor; MLXIPL; MONDOB; WBSCR 14; WBSCR14; Williams Beuren syndrome chromosome region 14; Williams Beuren syndrome chromosome region 14 protein; WS basic helix loop helix leucine zipper protein; WS bHLH; MLXPL_HUMAN. |
| Background | ChREBP (Carbohydrate responsive element binding protein) is a transcription factor playing a critical role in the nutrient and hormonal regulation of genes encoding enzymes of glucose metabolism and lipogenesis pathways. It contains several domains including a nuclear localization signal (NLS) near the N-terminus, polyproline domains, a basic helix-loop-helix leucine zipper (b/HLH/Zip) and a leucine zipper like (zip-like) domain.ChREBP is ubiquitously detected in various tissues, with highest expression in liver, kidney and white and brown adipose tissue. Under basal conditions ChREBP is localized in the cytosol, translocating into the nucleus upon high glucose stimulation following its dephosphorylation of serine 196. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is WBSCR14 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-3516R-APC-CY5-5) validated for, and at what dilution?
BS-3516R-APC-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does WBSCR14 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-3516R-APC-CY5-5) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Pig, Horse, Chicken.
How should BS-3516R-APC-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise WBSCR14 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-3516R-APC-CY5-5)?
KLH conjugated synthetic peptide derived from human WBSCR14/ChREBP
What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BS-3516R-APC-CY5-5)?
APC-Cy5.5 on BS-3516R-APC-CY5-5: excitation / emission 650nm/694nm.