SLK Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3519R-Cy5 |
| Product Name | SLK Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Pig, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SLK |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9748 |
| Subcellular location | Cell membrane |
| Synonyms | Fyn; C syn protooncogene; FYN oncogene related to SRC FGR YES_ MGC45350; OKT3 induced calcium influx regulator; P59 FYN; Protein tyrosine kinase fyn; Proto oncogene tyrosine protein kinase fyn; Protooncogene Syn; SLK; Src like kinase; Src yes related novel gene; Src/yes related novel gene;SYN; Tyrosine kinase p59fyn T; Tyrosine kinase p59fynT. |
| Background | Fyn is a membrane-associated, non-receptor protein tyrosine kinase approximately 59kDa, which belongs to the Src family of cytoplasmic tyrosine kinases. Fyn is very strongly similar to mouse Fyn, v-yes and c-src. Fyn is expressed predominately in tissues of neuronal and hematopoietic origin. Neuronal Fyn and hematopoietic Fyn differ at the junction of the SH2 and kinase domains due to tissue specific alternative splicing. Fyn has been shown to be involved in B cell and T cell activation as well as keratinocyte differentiation. In T cells, Fyn associates with the T-cell antigen receptor and Thy1.The unique N terminal domain of Fyn interacts with the CD3 and eta chains of the TcR. Fyn can bind to other proteins (p82 and p116) through its SH2 and SH3 domains, which may act as substrates or regulators of Fyn activity. Fyn is highly expressed in brain suggesting that it may have a role in the sensory nervous network and in myelination at early stages of CNS formation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SLK Polyclonal Antibody, Cy5 Conjugated (bs-3519R-Cy5) validated for, and at what dilution?
bs-3519R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SLK Polyclonal Antibody, Cy5 Conjugated (bs-3519R-Cy5) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Chicken, Rabbit.
How should bs-3519R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SLK Polyclonal Antibody, Cy5 Conjugated (bs-3519R-Cy5)?
KLH conjugated synthetic peptide derived from human SLK
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-3519R-Cy5)?
Cy5 on bs-3519R-Cy5: excitation / emission 625,650nm/670nm.