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PFKFB3/PFK2 Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Cow
  • Pig
  • Horse
  • Rabbit
  • GuineaPig
Overview
Catalog # bs-3528R-FITC
Product Name PFKFB3/PFK2 Polyclonal Antibody, FITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Cow, Pig, Horse, Rabbit, GuineaPig
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PFK2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5209
Swiss Prot Q16875
Subcellular location Cytoplasm, Nucleus
Synonyms PFK2; IPFK2; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3; 6PF-2-K/Fru-2,6-P2ase 3; PFK/FBPase 3; 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme; Renal carcinoma antigen NY-REN-56; iPFK-2; PFKFB3
Background Synthesis and degradation of fructose 2,6-bisphosphate.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is PFKFB3/PFK2 Polyclonal Antibody, FITC Conjugated (bs-3528R-FITC) validated for, and at what dilution?

bs-3528R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PFKFB3/PFK2 Polyclonal Antibody, FITC Conjugated (bs-3528R-FITC) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Pig, Horse, Rabbit, GuineaPig.

How should bs-3528R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PFKFB3/PFK2 Polyclonal Antibody, FITC Conjugated (bs-3528R-FITC)?

KLH conjugated synthetic peptide derived from human PFK2

What are the excitation and emission wavelengths of the FITC conjugate (bs-3528R-FITC)?

FITC on bs-3528R-FITC: excitation / emission 494nm/518nm.