DTNB Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3548R-100uL |
| Product Name | DTNB Polyclonal Antibody |
| Applications |
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This antibody may cross-react with DTNA based upon a 75% sequence similarity across the immunogen sequence range. |
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DTNB |
| Immunogen Range | 261-370/627 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 1838 |
| Swiss Prot | O60941 |
| Subcellular location | Cytoplasm |
| Synonyms | Dystrobrevin beta; DTN-B; Beta-dystrobrevin; DTNB |
| Background | Dystrobrevin beta is a component of the dystrophin-associated protein complex and interacts with dystrophin short form DP71, syntrophins SNTG1 and SNTG2. It localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. 4 isoforms produced by alternative splicing have been identified. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DTNB Polyclonal Antibody (bs-3548R) validated for, and at what dilution?
bs-3548R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DTNB Polyclonal Antibody (bs-3548R) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Horse, Chicken, Rabbit.
How should bs-3548R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DTNB Polyclonal Antibody (bs-3548R)?
KLH conjugated synthetic peptide derived from human DTNB Immunogen range: 261-370/627.