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DTNB Polyclonal Antibody, FITC Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Cow
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-3548R-FITC
Product Name DTNB Polyclonal Antibody, FITC Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity This antibody may cross-react with DTNA based upon a 75% sequence similarity across the immunogen sequence range.
Reactivity Rat
Predicted Reactivity Human, Mouse, Dog, Cow, Horse, Chicken, Rabbit
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human DTNB
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1838
Swiss Prot O60941
Subcellular location Cytoplasm
Synonyms Dystrobrevin beta; DTN-B; Beta-dystrobrevin; DTNB
Background Dystrobrevin beta is a component of the dystrophin-associated protein complex and interacts with dystrophin short form DP71, syntrophins SNTG1 and SNTG2. It localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. 4 isoforms produced by alternative splicing have been identified.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is DTNB Polyclonal Antibody, FITC Conjugated (bs-3548R-FITC) validated for, and at what dilution?

bs-3548R-FITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does DTNB Polyclonal Antibody, FITC Conjugated (bs-3548R-FITC) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Horse, Chicken, Rabbit.

How should bs-3548R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise DTNB Polyclonal Antibody, FITC Conjugated (bs-3548R-FITC)?

KLH conjugated synthetic peptide derived from human DTNB

What are the excitation and emission wavelengths of the FITC conjugate (bs-3548R-FITC)?

FITC on bs-3548R-FITC: excitation / emission 494nm/518nm.