Myogenin Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3550R-100uL |
| Product Name | Myogenin Polyclonal Antibody |
| Applications |
WB
IHC-P
IHC-F
IF
ICC/IF
|
| Reactivity | Human, Mouse, Rat, Bovine |
| Predicted Reactivity | Pig |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MYOG/Myogenin |
| Immunogen Range | 80-125/224 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 4656 |
| Swiss Prot | P15173 |
| Subcellular location | Nucleus |
| Synonyms | MYF4; myf-4; bHLHc3; Myogenin; Class C basic helix-loop-helix protein 3; Myogenic factor 4; MYOG |
| Background | Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| IHC-F | IFIHC-P1:50-200 |
| IF | |
| ICC/IF | |
Frequently asked questions
Which applications is Myogenin Polyclonal Antibody (bs-3550R) validated for, and at what dilution?
bs-3550R is validated for WB (1:300-5000); IHC-P (1:200-400); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Myogenin Polyclonal Antibody (bs-3550R) react with?
Tested reactivity: Human, Mouse, Rat, Bovine. Predicted reactivity (expected from sequence homology but not yet tested): Pig.
How should bs-3550R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Myogenin Polyclonal Antibody (bs-3550R)?
KLH conjugated synthetic peptide derived from human MYOG/Myogenin Immunogen range: 80-125/224.