Myogenin Polyclonal Antibody, PE Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3550R-PE-100uL |
| Product Name | Myogenin Polyclonal Antibody, PE Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat, Bovine |
| Predicted Reactivity | Pig |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MYOG/Myogenin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4656 |
| Swiss Prot | P15173 |
| Subcellular location | Nucleus |
| Synonyms | MYF4; myf-4; bHLHc3; Myogenin; Class C basic helix-loop-helix protein 3; Myogenic factor 4; MYOG |
| Background | Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is Myogenin Polyclonal Antibody, PE Conjugated (bs-3550R-PE) validated for, and at what dilution?
bs-3550R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Myogenin Polyclonal Antibody, PE Conjugated (bs-3550R-PE) react with?
Tested reactivity: Human, Mouse, Rat, Bovine. Predicted reactivity (expected from sequence homology but not yet tested): Pig.
How should bs-3550R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Myogenin Polyclonal Antibody, PE Conjugated (bs-3550R-PE)?
KLH conjugated synthetic peptide derived from human MYOG/Myogenin
What are the excitation and emission wavelengths of the PE conjugate (bs-3550R-PE)?
PE on bs-3550R-PE: excitation / emission 496,564nm/578nm.