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NUCB2 Polyclonal Antibody, PE-Cy5 Conjugated

Applications

  • FCM

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-3552R-PE-Cy5-100uL
Product Name NUCB2 Polyclonal Antibody, PE-Cy5 Conjugated
Applications
FCM
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation PE-Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Nesfatin-1/Nucb2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4925
Swiss Prot P80303
Subcellular location Cytoplasm, Nucleus, Secreted, Cell membrane
Synonyms NEFA; HEL-S-19; Nucleobindin-2; DNA-binding protein NEFA; Gastric cancer antigen Zg4; Prepronesfatin; NUCB2
Background Calcium-binding protein. May have a role in calcium homeostasis. Nesfatin-1: Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance (By similarity).
Application Dilution
FCM 1:20-100

Frequently asked questions

Which applications is NUCB2 Polyclonal Antibody, PE-Cy5 Conjugated (bs-3552R-PE-Cy5) validated for, and at what dilution?

bs-3552R-PE-Cy5 is validated for FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NUCB2 Polyclonal Antibody, PE-Cy5 Conjugated (bs-3552R-PE-Cy5) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, Rabbit.

How should bs-3552R-PE-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NUCB2 Polyclonal Antibody, PE-Cy5 Conjugated (bs-3552R-PE-Cy5)?

KLH conjugated synthetic peptide derived from human Nesfatin-1/Nucb2

What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-3552R-PE-Cy5)?

PE-Cy5 on bs-3552R-PE-Cy5: excitation / emission 488nm/670nm.

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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