ADAMTS5 Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Aggrecanase-2\/ADAMTS5 Polyclonal Antibody, Unconjugated (bs-3573R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Aggrecanase-2\/ADAMTS5 Polyclonal Antibody, Unconjugated (bs-3573R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Spleen tissue lysates; Lane 3: Human U87MG cell lysates; Lane 4: Human U251 cell lysates; Lane 5: Human SH-SY5Y cell lysates; Lane 6: Human HeLa cell lysates probed with ADAMTS5 Polyclonal Antibody, Unconjugated (bs-3573R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Spleen tissue lysates; Lane 3: Human U87MG cell lysates; Lane 4: Human U251 cell lysates; Lane 5: Human SH-SY5Y cell lysates; Lane 6: Human HeLa cell lysates probed with ADAMTS5 Polyclonal Antibody, Unconjugated (bs-3573R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
ELISA
IHC-P
IHC-F
IF

Reactivity

Human
Mouse

Predicted Reactivity

Dog
Cow
Pig
Horse
Rabbit

Overview
Catalog #	bs-3573R
Product Name	ADAMTS5 Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF
Reactivity	Human, Mouse
Predicted Reactivity	Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ADAMTS5
Immunogen Range	511-610/930
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	11096
Swiss Prot	Q9UNA0
Subcellular location	Cytoplasm, Nucleus
Synonyms	ADAM-TS 11; ADAM-TS 5; ADAM-TS5; ADAMTS-11; ADAMTS-5; ADAMTS11; ADMP-2; 9530092O11Rik; ADAMTS1; ASMP-2; ATS5_HUMAN; ADAMTS5; A disintegrin and metalloproteinase with thrombospondin motifs 11 (ADAM-TS 11 \| ADAMTS-11); Aggrecanase-2; 3.4.24.-; ADMP2; ATS5_MOUSE; Implantin; ADAM metallopeptidase with thrombospondin type 1 motif 5; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2)
Background	Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F
IF