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HBEGF Polyclonal Antibody, FITC Conjugated

Applications

  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-3576R-FITC-100uL
Product Name HBEGF Polyclonal Antibody, FITC Conjugated
Applications
IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human HB-EGF/DTSF
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1839
Swiss Prot Q99075
Subcellular location Secreted, Cell membrane
Synonyms DTR; DTS; DTSF; HEGFL; Proheparin-binding EGF-like growth factor; HBEGF
Background Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
Application Dilution
IF(ICC) 1:50-200

Frequently asked questions

Which applications is HBEGF Polyclonal Antibody, FITC Conjugated (bs-3576R-FITC) validated for, and at what dilution?

bs-3576R-FITC is validated for IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HBEGF Polyclonal Antibody, FITC Conjugated (bs-3576R-FITC) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit.

How should bs-3576R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HBEGF Polyclonal Antibody, FITC Conjugated (bs-3576R-FITC)?

KLH conjugated synthetic peptide derived from human HB-EGF/DTSF

What are the excitation and emission wavelengths of the FITC conjugate (bs-3576R-FITC)?

FITC on bs-3576R-FITC: excitation / emission 494nm/518nm.