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TCTP Polyclonal Antibody, AbBy Fluor-555 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
  • GuineaPig
Overview
Catalog # bs-3578R-BF555-100uL
Product Name TCTP Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit, GuineaPig
Specifications
Conjugation AbBy Fluor-555
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TCTP
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7178
Subcellular location Cytoplasm
Synonyms FLJ27337; Fortilin; Histamine releasing factor; Histamine-releasing factor; HRF; Immunoglobulin E-dependent; p02; p23; TCTP; TCTP_HUMAN; TPT1; Translationally controlled tumor protein; Translationally-controlled tumor protein; Tumor protein translationally controlled 1; tumor protein translationally-controlled 1; tumor protein, translationally-controlled 1.
Background TCTP is involved in calcium binding and microtubule stabilization. It is an immunoglobulin E dependent histamine-releasing factor produced by lymphocytes of atopic children and present in biologic fluids of allergic patients. Both human and mouse recombinant HRF proteins caused histamine release from human basophils of a subpopulation of donors (referenced from OMIM).
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is TCTP Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-3578R-BF555) validated for, and at what dilution?

bs-3578R-BF555 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TCTP Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-3578R-BF555) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit, GuineaPig.

How should bs-3578R-BF555 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TCTP Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-3578R-BF555)?

KLH conjugated synthetic peptide derived from human TCTP

What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-3578R-BF555)?

AbBy Fluor-555 on bs-3578R-BF555: excitation / emission 553nm/568nm.

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