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TLK1 Polyclonal Antibody, HRP Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
Overview
Catalog # bs-3583R-HRP
Product Name TLK1 Polyclonal Antibody, HRP Conjugated
Applications
WB ELISA IHC-P IHC-F
Reactivity Mouse, Rat
Predicted Reactivity Human
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TLK1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9874
Subcellular location Nucleus
Synonyms PKU beta; serine threonine protein kinase; Serine/threonine protein kinase tousled like 1; TLK1; Tousled like kinase 1.
Background TLK1 is a nuclear serine/threonine kinases that are potentially involved in the regulation of chromatin assembly. TLK1 is rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is TLK1 Polyclonal Antibody, HRP Conjugated (bs-3583R-HRP) validated for, and at what dilution?

bs-3583R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TLK1 Polyclonal Antibody, HRP Conjugated (bs-3583R-HRP) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.

How should bs-3583R-HRP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TLK1 Polyclonal Antibody, HRP Conjugated (bs-3583R-HRP)?

KLH conjugated synthetic peptide derived from human TLK1