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ESM1 Polyclonal Antibody

Lane 1: mouse brain lysates Lane 2: human colon carcinoma lysates probed with Anti ESM1 Polyclonal Antibody, Unconjugated (bs-3615R) at 1:200 in 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 20kD. Observed band size: 20kD
Lane 1: mouse brain lysates Lane 2: human colon carcinoma lysates probed with Anti ESM1 Polyclonal Antibody, Unconjugated (bs-3615R) at 1:200 in 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 20kD. Observed band size: 20kD
Formalin-fixed and human kidney tissue labeled with Anti-ESM1 Polyclonal Antibody, Unconjugated (bs-3615R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and human kidney tissue labeled with Anti-ESM1 Polyclonal Antibody, Unconjugated (bs-3615R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ESM1 Polyclonal Antibody, Unconjugated (bs-3615R) at 1:400 overnight at 4\u00b0C, followed by a FITC conjugated secondary antibody for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ESM1 Polyclonal Antibody, Unconjugated (bs-3615R) at 1:400 overnight at 4\u00b0C, followed by a FITC conjugated secondary antibody for 90 minutes, and DAPI for nuclei staining.
Lane 1: Mouse Heart tissue lysates; Lane 2: Rat Heart tissue lysates probed with ESM1 Polyclonal Antibody, Unconjugated (bs-3615R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Mouse Heart tissue lysates; Lane 2: Rat Heart tissue lysates probed with ESM1 Polyclonal Antibody, Unconjugated (bs-3615R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Human HUVEC cell lysates; Lane 2: Human U-2 OS cell lysates; Lane 3: Human A549 cell lysates probed with ESM1 Polyclonal Antibody, Unconjugated (bs-3615R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Human HUVEC cell lysates; Lane 2: Human U-2 OS cell lysates; Lane 3: Human A549 cell lysates probed with ESM1 Polyclonal Antibody, Unconjugated (bs-3615R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Cow
  • Pig
  • Horse
Overview
Catalog # bs-3615R
Product Name ESM1 Polyclonal Antibody
Applications
WB ELISA IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Cow, Pig, Horse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ESM1
Immunogen Range 51-150/184
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 11082
Swiss Prot Q9NQ30
Subcellular location Secreted
Synonyms endocan; Endothelial cell-specific molecule 1; ESM-1; ESM1
Background Involved in angiogenesis; promotes angiogenic sprouting. May have potent implications in lung endothelial cell-leukocyte interactions.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is ESM1 Polyclonal Antibody (bs-3615R) validated for, and at what dilution?

bs-3615R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ESM1 Polyclonal Antibody (bs-3615R) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Pig, Horse.

How should bs-3615R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ESM1 Polyclonal Antibody (bs-3615R)?

KLH conjugated synthetic peptide derived from human ESM1 Immunogen range: 51-150/184.

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