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HRH3 Polyclonal Antibody

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF(IHC-P)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Cow
  • Horse
Overview
Catalog # bs-3635R-100uL
Product Name HRH3 Polyclonal Antibody
Applications
WB IHC-P IHC-F IF(IHC-P)
Reactivity Rat
Predicted Reactivity Human, Mouse, Dog, Cow, Horse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human HRH3/GPCR97
Immunogen Range 121-220/445
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 11255
Swiss Prot Q9Y5N1
Subcellular location Cell membrane
Synonyms HH3R; GPCR97; Histamine H3 receptor; H3R; G-protein coupled receptor 97; HRH3
Background The H3 subclass of histamine receptors could mediate the histamine signals in CNS and peripheral nervous system. Signals through the inhibition of adenylate cyclase and displays high constitutive activity (spontaneous activity in the absence of agonist). Agonist stimulation of isoform 3 neither modified adenylate cyclase activity nor induced intracellular calcium mobilization.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is HRH3 Polyclonal Antibody (bs-3635R) validated for, and at what dilution?

bs-3635R is validated for WB (1:300-5000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HRH3 Polyclonal Antibody (bs-3635R) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Horse.

How should bs-3635R be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HRH3 Polyclonal Antibody (bs-3635R)?

KLH conjugated synthetic peptide derived from human HRH3/GPCR97 Immunogen range: 121-220/445.

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