Tetraspan 5 Polyclonal Antibody, Cy3 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-3637R-Cy3 |
| Product Name | Tetraspan 5 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Tetraspan 5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10098 |
| Subcellular location | Cell membrane |
| Synonyms | NET 4; TSPAN 5; NET4; Tetraspan 5; Tetraspan-5; Tetraspan NET 4; Tetraspan TM4SF; Tetraspanin 5; Tetraspanin5; Tetraspan5; Tspaw5; TM4SF9; Transmembrane 4 superfamily member 8; Transmembrane 4 superfamily member 9. |
| Background | TSPAN5 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Tetraspan 5 Polyclonal Antibody, Cy3 Conjugated (bs-3637R-Cy3) validated for, and at what dilution?
bs-3637R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-3637R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Tetraspan 5 Polyclonal Antibody, Cy3 Conjugated (bs-3637R-Cy3)?
KLH conjugated synthetic peptide derived from human Tetraspan 5
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-3637R-Cy3)?
Cy3 on bs-3637R-Cy3: excitation / emission 512,550nm/570,615nm.