MAGEA4 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-3645R-Cy3 |
| Product Name | MAGEA4 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MAGE-A4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4103 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | MAGE 4; MAGE 4 antigen; MAGE 41; MAGE 41 antigen; MAGE 4A; MAGE 4B; MAGE X2; MAGE X2 antigen; MAGE4; MAGE41; MAGE4A; MAGE4B; MAGEA 4; MAGEA4; Melanoma antigen family A 4; Melanoma associated antigen 4; Cancer/testis antigen 1.4; CT1.4. |
| Background | This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is MAGEA4 Polyclonal Antibody, Cy3 Conjugated (bs-3645R-Cy3) validated for, and at what dilution?
bs-3645R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MAGEA4 Polyclonal Antibody, Cy3 Conjugated (bs-3645R-Cy3) react with?
Tested reactivity: Human.
How should bs-3645R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MAGEA4 Polyclonal Antibody, Cy3 Conjugated (bs-3645R-Cy3)?
KLH conjugated synthetic peptide derived from human MAGE-A4
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-3645R-Cy3)?
Cy3 on bs-3645R-Cy3: excitation / emission 512,550nm/570,615nm.