Overview |
bs-3657R |
FLASH/CASP8AP2 Polyclonal Antibody |
WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC) |
Mouse |
Human, Rat, Dog, Cow, Pig, Horse |
Specifications |
Unconjugated |
Rabbit |
KLH conjugated synthetic peptide derived from human FLASH/CASP8AP2 |
1861-1982/1982 |
Polyclonal |
IgG |
1ug/ul |
Purified by Protein A. |
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Target |
9994 |
Cytoplasm, Nucleus |
CASP 8 associated protein 2; CASP8 associated protein 2; CASP8AP2; Caspase 8 associated protein 2; CED 4; CED4; FLASH homolog; FLASH homolog RIP 25; FLASH homolog RIP25; FLICE associated huge; FLICE associated huge protein; FLICE-associated huge protein; RIP 25; RIP25; AA387232; D4Ertd659e; mKIAA1315; C8AP2_HUMAN; CASP8-associated protein 2; FLICE-associated huge protein; FLJ11208; KIAA1315; OTTMUSP00000005125; RP23-69G24.1. |
FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8. There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity.Studies of FLASH protein suggested that this protein may be a component of the death inducing signaling complex that includes Fas receptor, Fas binding adapter FADD, and caspase 8, and plays a regulatory role in Fas mediated apoptosis. |
Application Dilution |
WB |
1:300-5000 |
ELISA |
1:500-1000 |
IHC-P |
1:200-400 |
IHC-F |
1:100-500 |
IF(IHC-P) |
1:50-200 |
IF(IHC-F) |
1:50-200 |
IF(ICC) |
1:50-200 |