NIPP1 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3658R-Cy5 |
| Product Name | NIPP1 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Zebrafish |
| Predicted Reactivity | Human, Rat, Cow, Pig, Horse, Chicken, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NIPP1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5511 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Activator of RNA decay; ARD 1; ARD1; Homolog of E.coli RNase E; NIPP 1; NIPP1; Nuclear inhibitor of protein phosphatase 1; Nuclear subunit of PP1; PPP1R8; Protein phosphatase 1 regulatory inhibitor subunit 8; Protein phosphatase 1 regulatory subunit 8; RNase E. |
| Background | Inhibitor subunit of the major nuclear protein phosphatase 1 (PP1). It has RNA binding activity but does not cleave RNA and may target PP1 to RNA associated substrates. May also be involved in pre mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NIPP1 Polyclonal Antibody, Cy5 Conjugated (bs-3658R-Cy5) validated for, and at what dilution?
bs-3658R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NIPP1 Polyclonal Antibody, Cy5 Conjugated (bs-3658R-Cy5) react with?
Tested reactivity: Mouse, Zebrafish. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Pig, Horse, Chicken, Rabbit, GuineaPig.
How should bs-3658R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NIPP1 Polyclonal Antibody, Cy5 Conjugated (bs-3658R-Cy5)?
KLH conjugated synthetic peptide derived from human NIPP1
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-3658R-Cy5)?
Cy5 on bs-3658R-Cy5: excitation / emission 625,650nm/670nm.