DACH2 Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-3659R-BF555-100uL |
| Product Name | DACH2 Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DACH2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 117154 |
| Subcellular location | Nucleus |
| Synonyms | DACH 2; dachshund 2; Dachshund homolog 2 Drosophila; Dachshund homolog 2; Dachshund2; FLJ31391; HGNC:16814; MGC138545. |
| Background | DACH2 is a transcription factor that is involved in regulation of organogenesis. It seems to be a regulator for SIX1 and SIX6 and may act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. It probably binds to DNA via its DACH box-N domain. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DACH2 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-3659R-BF555) validated for, and at what dilution?
bs-3659R-BF555 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-3659R-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DACH2 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-3659R-BF555)?
KLH conjugated synthetic peptide derived from human DACH2
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-3659R-BF555)?
AbBy Fluor-555 on bs-3659R-BF555: excitation / emission 553nm/568nm.