RALDH2 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Anti-RALDH2 Polyclonal Antibody, Unconjugated (bs-3676R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin-embedded rat brain labeled with Anti-RALDH2 Polyclonal Antibody, Unconjugated(bs-3676R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG,Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.\\n$

Formalin-fixed and paraffin-embedded rat brain labeled with Anti-RALDH2 Polyclonal Antibody, Unconjugated(bs-3676R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG,Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.\\n

$Formalin-fixed and paraffin-embedded mouse embryo labeled with Anti-RALDH2 Polyclonal Antibody, Unconjugated(bs-3676R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG,Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.\\n$

Formalin-fixed and paraffin-embedded mouse embryo labeled with Anti-RALDH2 Polyclonal Antibody, Unconjugated(bs-3676R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG,Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.\\n

Lane 1: Mouse Testis tissue lysates; Lane 2: Rat Testis tissue lysates; Lane 3: Human K562 cell lysates; Lane 4: Mouse Uterus tissue lysates probed with RALDH2 Polyclonal Antibody, Unconjugated (bs-3676R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (RALDH2) Polyclonal Antibody, Unconjugated (bs-3676R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (RALDH2) Polyclonal Antibody, Unconjugated (bs-3676R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse
Rat
Zebrafish

Predicted Reactivity

Human
Dog
Cow
Pig
Horse
Rabbit

Overview
Catalog #	bs-3676R
Product Name	RALDH2 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Mouse, Rat, Zebrafish
Predicted Reactivity	Human, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human RALDH2
Immunogen Range	441-518/518
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8854
Swiss Prot	O94788
Subcellular location	Cytoplasm
Synonyms	RALDH2; RALDH2-T; RALDH(II); Retinal dehydrogenase 2; RALDH 2; Aldehyde dehydrogenase family 1 member A2; Retinaldehyde-specific dehydrogenase type 2; ALDH1A2
Background	Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Does metabolize octanal and decanal but does not metabolize citral, benzaldehyde, acetaldehyde and propanal efficiently (By similarity).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200

Applications

Reactivity

Predicted Reactivity

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