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Adenylate cyclase 1 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Anti-ADCY1 Polyclonal Antibody, Unconjugated (bs-3681R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-ADCY1 Polyclonal Antibody, Unconjugated (bs-3681R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
A549 cell lysates probed with ADCY1 Polyclonal Antibody, Unconjugated (bs-3681R) at 1:300 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
A549 cell lysates probed with ADCY1 Polyclonal Antibody, Unconjugated (bs-3681R) at 1:300 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
MCF-7 cell lysates probed with ADCY1 Polyclonal Antibody, Unconjugated (bs-3681R) at 1:300 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
MCF-7 cell lysates probed with ADCY1 Polyclonal Antibody, Unconjugated (bs-3681R) at 1:300 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Paraformaldehyde-fixed, paraffin embedded Rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ADCY1 Polyclonal Antibody, Unconjugated (bs-3681R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ADCY1 Polyclonal Antibody, Unconjugated (bs-3681R) at 1:200 overnight at 4\u00b0C, DAB staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
Overview
Catalog # bs-3681R
Product Name Adenylate cyclase 1 Polyclonal Antibody
Applications
WB IHC-P IHC-F IF
Reactivity Human, Rat
Predicted Reactivity Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Adenylyl cyclase
Immunogen Range 751-850/1119
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 107
Swiss Prot Q08828
Subcellular location Cell membrane
Synonyms AC1; DFNB44; Adenylate cyclase type 1; ATP pyrophosphate-lyase 1; Adenylate cyclase type I; Adenylyl cyclase 1; Ca(2+)/calmodulin-activated adenylyl cyclase; ADCY1
Background This is a calmodulin-sensitive adenylyl cyclase. May be involved in regulatory processes in the central nervous system. It may play a role in memory acquisition and learning. Plays a role in the regulation of the circadian rhythm of daytime contrast sensitivity probably by modulating the rhythmic synthesis of cyclic AMP in the retina (By similarity).
Application Dilution
WB 1:300-5000
IHC-P ELISA1:500-1000
IHC-F IHC-P1:200-400
IF

Frequently asked questions

Which applications is Adenylate cyclase 1 Polyclonal Antibody (bs-3681R) validated for, and at what dilution?

bs-3681R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Adenylate cyclase 1 Polyclonal Antibody (bs-3681R) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse.

How should bs-3681R be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Adenylate cyclase 1 Polyclonal Antibody (bs-3681R)?

KLH conjugated synthetic peptide derived from human Adenylyl cyclase Immunogen range: 751-850/1119.

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