OGG1 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3687R-RBITC-100uL |
| Product Name | OGG1 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human OGG1/8 hydroxyguanine DNA glycosylase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4968 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | 8 hydroxyguanine DNA glycosylase; 8 oxoguanine DNA glycosylase 1; 8-oxoguanine DNA glycosylase; AP lyase; DNA apurinic or apyrimidinic site lyase; DNA lyase; DNA-apurinic or apyrimidinic site lyase; HMMH; HOGG 1; HOGG1; MMH; MUTM; N-glycosylase; Ogg 1; OGH 1; OGH1; OGG1_HUMAN. |
| Background | This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined. [provided by RefSeq, Aug 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is OGG1 Polyclonal Antibody, RBITC Conjugated (bs-3687R-RBITC) validated for, and at what dilution?
bs-3687R-RBITC is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does OGG1 Polyclonal Antibody, RBITC Conjugated (bs-3687R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig.
How should bs-3687R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise OGG1 Polyclonal Antibody, RBITC Conjugated (bs-3687R-RBITC)?
KLH conjugated synthetic peptide derived from human OGG1/8 hydroxyguanine DNA glycosylase
What are the excitation and emission wavelengths of the RBITC conjugate (bs-3687R-RBITC)?
RBITC on bs-3687R-RBITC: excitation / emission 540nm/625nm.