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GPR15 Polyclonal Antibody, PerCP Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Horse
  • Rabbit
Overview
Catalog # bs-3699R-PerCP
Product Name GPR15 Polyclonal Antibody, PerCP Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Horse, Rabbit
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GPR15
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2838
Swiss Prot P49685
Subcellular location Cytoplasm, Cell membrane
Synonyms BOB; G-protein coupled receptor 15; Brother of Bonzo; GPR15
Background GPR15 is a probable chemokine receptor,its expression has been reported in CD4(+) T lymphocytes, alveolar macrophages, and the basal surface of the small intestinal epithelium. ESTs have been isolated from normal brain and kidney cancer libraries.This gene encodes a G protein-coupled receptor that acts as a chemokine receptor for human immunodeficiency virus type 1 and 2. The encoded protein localizes to the cell membrane. [provided by RefSeq, Nov 2012].
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is GPR15 Polyclonal Antibody, PerCP Conjugated (bs-3699R-PerCP) validated for, and at what dilution?

bs-3699R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GPR15 Polyclonal Antibody, PerCP Conjugated (bs-3699R-PerCP) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Horse, Rabbit.

How should bs-3699R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GPR15 Polyclonal Antibody, PerCP Conjugated (bs-3699R-PerCP)?

KLH conjugated synthetic peptide derived from human GPR15

What are the excitation and emission wavelengths of the PerCP conjugate (bs-3699R-PerCP)?

PerCP on bs-3699R-PerCP: excitation / emission 482nm/677nm.