phospho-P53 (Ser315) Polyclonal Antibody, APC-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-3704R-APC-Cy5.5-100uL |
| Product Name | phospho-P53 (Ser315) Polyclonal Antibody, APC-Cy5.5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | APC-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human P53 around the phosphorylation site of Ser315 |
| Modification Site | Ser315 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7157 |
| Swiss Prot | P04637 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | P53; BCC7; LFS1; TRP53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53 |
| Background | Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492). |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is phospho-P53 (Ser315) Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-3704R-APC-CY5-5) validated for, and at what dilution?
BS-3704R-APC-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-P53 (Ser315) Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-3704R-APC-CY5-5) react with?
Tested reactivity: Human, Mouse.
How should BS-3704R-APC-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-P53 (Ser315) Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-3704R-APC-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from human P53 around the phosphorylation site of Ser315
What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BS-3704R-APC-CY5-5)?
APC-Cy5.5 on BS-3704R-APC-CY5-5: excitation / emission 650nm/694nm.