phospho-P53 (Thr81) Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3711R-BF555-100uL |
| Product Name | phospho-P53 (Thr81) Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human P53 around the phosphorylation site of Thr81 |
| Modification Site | Thr81 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7157 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | p53 Thr81; P53 phospho T81; P53 phospho Thr81; p-P53 Thr81; p-P53 T81; Widespread p53; Wtp53; Antigen NY-CO-13; Cellular tumor antigen p53; Cys 51 Stop; HGNC11998; LFS1; p53; p53 Cellular Tumor Antigen; p53 Tumor Suppressor; Phosphoprotein p53; TP53; Transformation related protein 53; TRP53; Tumor protein p53; Tumour Protein p53; P53_HUMAN; TP53. |
| Background | This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013]. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-P53 (Thr81) Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-3711R-BF555) validated for, and at what dilution?
bs-3711R-BF555 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-P53 (Thr81) Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-3711R-BF555) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Chicken.
How should bs-3711R-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-P53 (Thr81) Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-3711R-BF555)?
KLH conjugated synthetic phosphopeptide derived from human P53 around the phosphorylation site of Thr81
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-3711R-BF555)?
AbBy Fluor-555 on bs-3711R-BF555: excitation / emission 553nm/568nm.