Phospho-PKA alpha + beta (Thr198) Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3725R-BF405-100uL |
| Product Name | Phospho-PKA alpha + beta (Thr198) Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Pig, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human PRKACB around the phosphorylation site of Thr198 |
| Modification Site | Thr198 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5567 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | PKA alpha + betaThr198; C alpha; cAMP dependent protein kinase beta catalytic subunit; cAMP dependent protein kinase alpha catalytic subunit; cAMP dependent protein kinase catalytic subunit alpha; cAMP dependent protein kinase catalytic subunit beta; Cs; PKA; PKA C; PKA C alpha; PKA C beta; PKACA; PKACB; PRKACA; PRKACB; Protein kinase cAMP dependent catalytic alpha; Protein kinase cAMP dependent catalytic beta; Protein kinase cAMP dependent catalytic beta isoform a; Protein kinase cAMP dependent catalytic beta isoform b. |
| Background | PRKACA and PRKACB are members of the Ser/Thr protein kinase family and are a catalytic subunit of cAMP-dependent protein kinase. cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.PKA alpha + beta (catalytic subunits) (phospho Thr198) |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-PKA alpha + beta (Thr198) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-3725R-BF405) validated for, and at what dilution?
bs-3725R-BF405 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-PKA alpha + beta (Thr198) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-3725R-BF405) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Pig, Chicken.
How should bs-3725R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-PKA alpha + beta (Thr198) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-3725R-BF405)?
KLH conjugated synthetic phosphopeptide derived from human PRKACB around the phosphorylation site of Thr198
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-3725R-BF405)?
AbBy Fluor-405 on bs-3725R-BF405: excitation / emission 401nm/421nm.