phospho-PKC delta (Tyr311) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-3728R-Cy3 |
| Product Name | phospho-PKC delta (Tyr311) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylation site is homologous to that of Tyr311 in Mouse and Rat. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human PKC delta around the phosphorylation site of Tyr313 |
| Modification Site | Tyr313 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5580 |
| Swiss Prot | Q05655 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | MAY1; PKCD; ALPS3; CVID9; nPKC-delta; Protein kinase C delta type; Tyrosine-protein kinase PRKCD; PRKCD |
| Background | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-PKC delta (Tyr311) Polyclonal Antibody, Cy3 Conjugated (bs-3728R-Cy3) validated for, and at what dilution?
bs-3728R-Cy3 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-PKC delta (Tyr311) Polyclonal Antibody, Cy3 Conjugated (bs-3728R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-3728R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-PKC delta (Tyr311) Polyclonal Antibody, Cy3 Conjugated (bs-3728R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human PKC delta around the phosphorylation site of Tyr313
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-3728R-Cy3)?
Cy3 on bs-3728R-Cy3: excitation / emission 512,550nm/570,615nm.