Phospho-Cyclin B1 (Ser126) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-3733R-Cy5 |
| Product Name | Phospho-Cyclin B1 (Ser126) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Ser126 |
| Modification Site | Ser126 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 891 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Cyclin B1 phospho S126; Cyclin B1 Ser126; p-Cyclin B1 S126; p-Cyclin B1 Ser126; p-Cyclin B1 phospho S126; CCNB 1; CCNB; CCNB1; CCNB1_HUMAN; G2 mitotic specic cyclin B1; G2/mitotic-specic cyclin-B1. |
| Background | The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-Cyclin B1 (Ser126) Polyclonal Antibody, Cy5 Conjugated (bs-3733R-Cy5) validated for, and at what dilution?
bs-3733R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Cyclin B1 (Ser126) Polyclonal Antibody, Cy5 Conjugated (bs-3733R-Cy5) react with?
Tested reactivity: Human.
How should bs-3733R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Cyclin B1 (Ser126) Polyclonal Antibody, Cy5 Conjugated (bs-3733R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Ser126
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-3733R-Cy5)?
Cy5 on bs-3733R-Cy5: excitation / emission 625,650nm/670nm.