phospho-DNA PKcs (Thr2609) Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-3735R-Cy5.5-100uL |
| Product Name | phospho-DNA PKcs (Thr2609) Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human PRKDC around the phosphorylation site of Thr2609 |
| Modification Site | Thr2609 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5591 |
| Subcellular location | Nucleus |
| Synonyms | DNA PKcsThr2609; PRKDCphospho T2609; DNA PKcs phospho T2609; p-DNA PKcs phospho T2609; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNPK1; p460; PRKDC; HYRC; HYRC1; Variant; DNAPK; DNAPK catalytic subunit; p350; PRKDC; Protein Kinase DNA Activated Catalytic Polypeptide; XRCC 7; XRCC7; PRKDC_HUMAN. |
| Background | DNA PK is the key component of the non-homologous end-joining (NHEJ) pathway of DSB repair in mammalian cells. DNA PK consists of a heterodimeric DNA-binding subunit (Ku70/80) and an approximately 465 kDa catalytic subunit (DNA PKcs). DNA PKcs is a serine/threonine protein kinase whose activity is greatly stimulated by its recruitment to DNA breaks by the Ku heterodimer. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-DNA PKcs (Thr2609) Polyclonal Antibody, Cy5.5 Conjugated (BS-3735R-CY5-5) validated for, and at what dilution?
BS-3735R-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-DNA PKcs (Thr2609) Polyclonal Antibody, Cy5.5 Conjugated (BS-3735R-CY5-5) react with?
Tested reactivity: Human, Mouse.
How should BS-3735R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-DNA PKcs (Thr2609) Polyclonal Antibody, Cy5.5 Conjugated (BS-3735R-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from human PRKDC around the phosphorylation site of Thr2609
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-3735R-CY5-5)?
Cy5.5 on BS-3735R-CY5-5: excitation / emission 675nm/694nm.