phospho-Claudin 6 (Tyr219) Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-3736R-PE-Cy5-100uL |
| Product Name | phospho-Claudin 6 (Tyr219) Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Horse, Rabbit |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Claudin 6 around the phosphorylation site of Tyr219 |
| Modification Site | Tyr219 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9074 |
| Subcellular location | Cell membrane |
| Synonyms | Claudin6; CLDN 6; CLDN6; Skullin 2; CLD6_HUMAN. |
| Background | Claudin 6 is a multipass transmembrane protein in the Claudin family. Claudin 6 is expressed by epithelial cells where it participates in tissue development and the maintenance of tight junction integrity. Claudin 6 is also a global marker of definitive endoderm. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is phospho-Claudin 6 (Tyr219) Polyclonal Antibody, PE-Cy5 Conjugated (bs-3736R-PE-Cy5) validated for, and at what dilution?
bs-3736R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-3736R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Claudin 6 (Tyr219) Polyclonal Antibody, PE-Cy5 Conjugated (bs-3736R-PE-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human Claudin 6 around the phosphorylation site of Tyr219
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-3736R-PE-Cy5)?
PE-Cy5 on bs-3736R-PE-Cy5: excitation / emission 488nm/670nm.