phospho-Crkl (Tyr251) Polyclonal Antibody, PE-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3738R-PE-Cy7-100uL |
| Product Name | phospho-Crkl (Tyr251) Polyclonal Antibody, PE-Cy7 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | PE-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Crkl around the phosphorylation site of Tyr251 [CA(p-Y)D] |
| Modification Site | Tyr251 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1399 |
| Swiss Prot | P46109 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Crk-like protein; CRKL |
| Background | Crkl is a 34kDa adaptor protein which has been shown to activate the RAS and JUN kinase signalling pathways and transforms fibroblasts in a RAS dependant manner. Crkl is a substitute of BCR ABL tyrosine kinase. In addition, Crkl has oncogenic potential. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is phospho-Crkl (Tyr251) Polyclonal Antibody, PE-Cy7 Conjugated (bs-3738R-PE-Cy7) validated for, and at what dilution?
bs-3738R-PE-Cy7 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Crkl (Tyr251) Polyclonal Antibody, PE-Cy7 Conjugated (bs-3738R-PE-Cy7) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-3738R-PE-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Crkl (Tyr251) Polyclonal Antibody, PE-Cy7 Conjugated (bs-3738R-PE-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human Crkl around the phosphorylation site of Tyr251 [CA(p-Y)D]
What are the excitation and emission wavelengths of the PE-Cy7 conjugate (bs-3738R-PE-Cy7)?
PE-Cy7 on bs-3738R-PE-Cy7: excitation / emission 488nm/780nm.