HSF1(Ser326) Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-3741r-100uL |
| Product Name | HSF1(Ser326) Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human HSF1 around the phosphorylation site of Ser326 |
| Modification Site | Ser326 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 3297 |
| Swiss Prot | Q00613 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | HSTF1; Heat shock factor protein 1; HSF 1; Heat shock transcription factor 1; HSTF 1; HSF1 |
| Background | DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HSF1(Ser326) Polyclonal Antibody (bs-3741R) validated for, and at what dilution?
bs-3741R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HSF1(Ser326) Polyclonal Antibody (bs-3741R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-3741R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HSF1(Ser326) Polyclonal Antibody (bs-3741R)?
KLH conjugated synthetic phosphopeptide derived from human HSF1 around the phosphorylation site of Ser326