Phospho-Paxillin (Tyr31) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3744R-Cy3 |
| Product Name | Phospho-Paxillin (Tyr31) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | A mismatch around the phosphorylation site decreases the antibody's affinity in Mouse/Rat samples |
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Cow, Sheep, Horse, Chicken |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Paxillin around the phosphorylation site of Tyr31 |
| Modification Site | Tyr31 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5829 |
| Swiss Prot | P49023 |
| Subcellular location | Cytoplasm |
| Synonyms | PXNphospho Y31; Paired box protein Pax 1; PAX 1; PAX1; Paxillin alpha; PXN; PXN protein; FLJ16691; FLJ23042; PAXI_HUMAN. |
| Background | Paxillin is a 64 kDa cytoskeletal adapter protein involved in organisation and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk). |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-Paxillin (Tyr31) Polyclonal Antibody, Cy3 Conjugated (bs-3744R-Cy3) validated for, and at what dilution?
bs-3744R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Paxillin (Tyr31) Polyclonal Antibody, Cy3 Conjugated (bs-3744R-Cy3) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Sheep, Horse, Chicken.
How should bs-3744R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Paxillin (Tyr31) Polyclonal Antibody, Cy3 Conjugated (bs-3744R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human Paxillin around the phosphorylation site of Tyr31
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-3744R-Cy3)?
Cy3 on bs-3744R-Cy3: excitation / emission 512,550nm/570,615nm.