phospho-HSF1 (Ser326) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-3745R-Cy3 |
| Product Name | phospho-HSF1 (Ser326) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(ICC)
IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated Synthesised phosphopeptide derived from human HSF1 around the phosphorylation site of Ser326 |
| Modification Site | Ser326 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3297 |
| Swiss Prot | Q00613 |
| Synonyms | HSF1 (phospho S326); p-HSF1 (phospho S326); Heat shock factor 1; Heat shock factor protein 1; Heat shock transcription factor 1; HSF 1; hsf1; HSTF 1; HSTF1. |
| Background | DNA-binding protein that specifically binds heat shockpromoter elements (HSE) and activates transcription. In highereukaryotes, HSF is unable to bind to the HSE unless the cells areheat shocked. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IF | |
Frequently asked questions
Which applications is phospho-HSF1 (Ser326) Polyclonal Antibody, Cy3 Conjugated (bs-3745R-Cy3) validated for, and at what dilution?
bs-3745R-Cy3 is validated for WB (1:300-5000); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-HSF1 (Ser326) Polyclonal Antibody, Cy3 Conjugated (bs-3745R-Cy3) react with?
Tested reactivity: Human.
How should bs-3745R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-HSF1 (Ser326) Polyclonal Antibody, Cy3 Conjugated (bs-3745R-Cy3)?
KLH conjugated Synthesised phosphopeptide derived from human HSF1 around the phosphorylation site of Ser326
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-3745R-Cy3)?
Cy3 on bs-3745R-Cy3: excitation / emission 512,550nm/570,615nm.