Histone H3 (acetyl K9, phospho S10) Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3748R-BF488 |
| Product Name | Histone H3 (acetyl K9, phospho S10) Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Cow, Pig, Rabbit, Fruit Fly |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Acetyl and Histone H3 (Ac-Lys10/p-Ser11) |
| Modification Site | Ac-K10/p-Ser11 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Nucleus |
| Synonyms | H3 histone family member E pseudogene; H3F3; HIST3H3; Histone H3 3 pseudogene. |
| Background | Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Histone H3 (acetyl K9, phospho S10) Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-3748R-BF488) validated for, and at what dilution?
bs-3748R-BF488 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Histone H3 (acetyl K9, phospho S10) Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-3748R-BF488) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Cow, Pig, Rabbit, Fruit Fly.
How should bs-3748R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Histone H3 (acetyl K9, phospho S10) Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-3748R-BF488)?
KLH conjugated synthetic peptide derived from human Acetyl and Histone H3 (Ac-Lys10/p-Ser11)
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-3748R-BF488)?
AbBy Fluor-488 on bs-3748R-BF488: excitation / emission 499nm/519nm.