Histone H3 (Tri Methyl K27) Antibody, PE-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3773R-PE-Cy5.5-100uL |
| Product Name | Histone H3 (Tri Methyl K27) Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Sheep, Pig, Rabbit |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic methyl peptide derived from human Histone H3 around methylation site of Lys27 |
| Modification Site | Tri Methyl K27 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3020 |
| Subcellular location | Nucleus |
| Synonyms | Histone H3, H3 histone family member E pseudogene; H3F3; HIST3H3; Histone H3 3 pseudogene. |
| Background | Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Histone H3 (Tri Methyl K27) Antibody, PE-Cy5.5 Conjugated (BS-3773R-PE-CY5-5) validated for, and at what dilution?
BS-3773R-PE-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Histone H3 (Tri Methyl K27) Antibody, PE-Cy5.5 Conjugated (BS-3773R-PE-CY5-5) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Rabbit.
How should BS-3773R-PE-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Histone H3 (Tri Methyl K27) Antibody, PE-Cy5.5 Conjugated (BS-3773R-PE-CY5-5)?
KLH conjugated synthetic methyl peptide derived from human Histone H3 around methylation site of Lys27
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BS-3773R-PE-CY5-5)?
PE-Cy5.5 on BS-3773R-PE-CY5-5: excitation / emission 488nm/694nm.