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GM-CSF Polyclonal Antibody

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti GM-CSF Polyclonal Antibody, Unconjugated (bs-3790R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti GM-CSF Polyclonal Antibody, Unconjugated (bs-3790R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Recombined Trx\/His-tag porcine GM-CSF protein; Lane 2: Recombined His-tag porcine GM-CSF protein probed with GM-CSF Polyclonal Antibody, Unconjugated (bs-3790R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Recombined Trx\/His-tag porcine GM-CSF protein; Lane 2: Recombined His-tag porcine GM-CSF protein probed with GM-CSF Polyclonal Antibody, Unconjugated (bs-3790R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Cow
  • Chicken
  • Rabbit
Overview
Catalog # bs-3790R
Product Name GM-CSF Polyclonal Antibody
Applications WB, ELISA, IHC-P, IHC-F, IF
Predicted Reactivity Human, Mouse, Rat, Dog, Cow, Chicken, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GM-CSF
Immunogen Range 65-144/144
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 1437
Swiss Prot P04141
Subcellular location Secreted
Synonyms Granulocyte-Macrophage Colony Stimulating Factor; Burst Promoting Activity; CMCSF; Colony Stimulating Factor 2; Colony stimulating factor; CSF 2; CSF Alpha; CSF; CSF2; Eosinophil Colony Stimulating Factor; GMCSF; Granulocyte Macrophage Colony Stimulating Factor; MGC131935; MGC138897; Molgramostin; Pluripoietin Alpha; Sargramostim; granulocyte-macrophage colony-stimulating factor precursor; CSF2_HUMAN.
Background Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Application Dilution
WB 1:500-2000
ELISA 1:5000-10000
IHC-P 1:100-500
IHC-F 1:100-500
IF 1:100-500

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