Help

GCDGP15 Polyclonal Antibody, Cy3 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
Overview
Catalog # bs-3795R-Cy3
Product Name GCDGP15 Polyclonal Antibody, Cy3 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GCDGP15/SABP
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5304
Subcellular location Secreted
Synonyms GCDGP15; GCDGP 15; GCDGP-15; Gp17; GPIP4; Gross cystic disease fluid protein 15; Prolactin induced protein; Prolactin inducible protein precursor; SABP; Secretory actin binding protein.PIP_HUMAN
Background Gross cystic disease is a common premenopausal disorder in which gross cysts are the predominant pathologic lesion. It is characterized by production of a fluid secretion which accumulates in the breast cysts. Gross cystic disease fluid is a pathologic secretion from breast composed of several glycoproteins, including a unique 15 kDa monomer protein, GCDFP 15. The cells within the body that produce GCDFP 15 appear to be restricted primarily to those with apocrine function. Studies have found GCDFP 15 to be a highly specific and sensitive marker for breast cancer.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is GCDGP15 Polyclonal Antibody, Cy3 Conjugated (bs-3795R-Cy3) validated for, and at what dilution?

bs-3795R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GCDGP15 Polyclonal Antibody, Cy3 Conjugated (bs-3795R-Cy3) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.

How should bs-3795R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GCDGP15 Polyclonal Antibody, Cy3 Conjugated (bs-3795R-Cy3)?

KLH conjugated synthetic peptide derived from human GCDGP15/SABP

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-3795R-Cy3)?

Cy3 on bs-3795R-Cy3: excitation / emission 512,550nm/570,615nm.