S-layer protein Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-3797R-RBITC-100uL |
| Product Name | S-layer protein Polyclonal Antibody, RBITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Bacteria |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from L, acidophilus S-layer protein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3252774 |
| Swiss Prot | P35829 |
| Subcellular location | Secreted |
| Synonyms | S-layer; Surface layer; surface layer protein; Slp; SLAP_LACAC; S-layer protein; slpA; slpH; Lactobacillus surface layer protein; |
| Background | One of the most fascinating cell envelope structures in prokaryotic organisms is two-dimensional arrays of protein or glycoprotein subunits, termed S-layers (Surface Layers). They are composed of numerous identical subunits forming a symmetric, porous, lattice-like layer that completely covers the cell surface. The subunits are held together and attached to cell wall carbohydrates by non-covalent interactions, and they spontaneously reassemble in vitro by an entropy-driven process. In lactobacilli, S-layer proteins have been detected on many but not all species. Lactobacillus S-layer proteins differ from those of other bacteria in their smaller size and high predicted pI. The positive charge in Lactobacillus S-layer proteins is concentrated in the more conserved cell wall binding domain, which can be either N- or C-terminal depending on the species. The more variable domain is responsible for the self-assembly of the monomers to a periodic structure. The biological functions of Lactobacillus S-layer proteins are poorly understood, but in some species S-layer proteins mediate bacterial adherence to host cells or extracellular matrix proteins or have protective or enzymatic functions. Lactobacillus S-layer proteins show potential for use as antigen carriers in live oral vaccine design because of their adhesive and immunomodulatory properties and the general non-pathogenicity of the species. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is S-layer protein Polyclonal Antibody, RBITC Conjugated (bs-3797R-RBITC) validated for, and at what dilution?
bs-3797R-RBITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does S-layer protein Polyclonal Antibody, RBITC Conjugated (bs-3797R-RBITC) react with?
Tested reactivity: Bacteria.
How should bs-3797R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise S-layer protein Polyclonal Antibody, RBITC Conjugated (bs-3797R-RBITC)?
KLH conjugated synthetic peptide derived from L, acidophilus S-layer protein
What are the excitation and emission wavelengths of the RBITC conjugate (bs-3797R-RBITC)?
RBITC on bs-3797R-RBITC: excitation / emission 540nm/625nm.