Oct-4B/OCT4B-190 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3816r-cy3-100uL |
| Product Name | Oct-4B/OCT4B-190 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Pig |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human OCT4B-190 |
| Immunogen Range | 201-300/360 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5460 |
| Swiss Prot | Q01860 |
| Subcellular location | Nucleus |
| Synonyms | OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4; POU domain, class 5, transcription factor 1; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; POU5F1 |
| Background | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP26. Critical for early embryogenesis and for embryonic stem cell pluripotency. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF | 1:100-500 |
Frequently asked questions
Which applications is Oct-4B/OCT4B-190 Polyclonal Antibody, Cy3 Conjugated (bs-3816R-Cy3) validated for, and at what dilution?
bs-3816R-Cy3 is validated for WB (1:500-2000); IF (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Oct-4B/OCT4B-190 Polyclonal Antibody, Cy3 Conjugated (bs-3816R-Cy3) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig.
How should bs-3816R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Oct-4B/OCT4B-190 Polyclonal Antibody, Cy3 Conjugated (bs-3816R-Cy3)?
KLH conjugated synthetic peptide derived from human OCT4B-190 Immunogen range: 201-300/360.
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-3816R-Cy3)?
Cy3 on bs-3816R-Cy3: excitation / emission 512,550nm/570,615nm.