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MrpL12 Polyclonal Antibody, RBITC Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
Overview
Catalog # bs-3834R-RBITC-100uL
Product Name MrpL12 Polyclonal Antibody, RBITC Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human MrpL-12
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6182
Subcellular location Cytoplasm
Synonyms 39S ribosomal protein L12, mitochondrial; 5c5-2; L12mt; MRP-L31/34; MRPL12; MRPL7/L12; RPML12; RM12_HUMAN.
Background MRPL12 is a component of the 39S subunit of mammalian mitochondrial ribosomes. This protein forms homodimers whereas in prokaryotic ribosomes two L7/L12 dimers and one L10 protein form the L8 protein complex.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is MrpL12 Polyclonal Antibody, RBITC Conjugated (bs-3834R-RBITC) validated for, and at what dilution?

bs-3834R-RBITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-3834R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise MrpL12 Polyclonal Antibody, RBITC Conjugated (bs-3834R-RBITC)?

KLH conjugated synthetic peptide derived from human MrpL-12

What are the excitation and emission wavelengths of the RBITC conjugate (bs-3834R-RBITC)?

RBITC on bs-3834R-RBITC: excitation / emission 540nm/625nm.

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