PABP (Methyl Arg455/460) Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3836R-RBITC |
| Product Name | PABP (Methyl Arg455/460) Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Cow, Pig, Horse, Chicken, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic metylpeptide derived from human PABP around metylation site of Arg455/Arg460 |
| Modification Site | Methyl Arg455/460 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 26986 |
| Swiss Prot | P11940 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | PAB1; PABP; PABP1; PABPC2; PABPL1; Polyadenylate-binding protein 1; PABP-1; Poly(A)-binding protein 1; PABPC1 |
| Background | Binds the poly(A) tail of mRNA, including that of its own transcript. May be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre-mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PABP (Methyl Arg455/460) Antibody, RBITC Conjugated (bs-3836R-RBITC) validated for, and at what dilution?
bs-3836R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PABP (Methyl Arg455/460) Antibody, RBITC Conjugated (bs-3836R-RBITC) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Pig, Horse, Chicken, Rabbit, GuineaPig.
How should bs-3836R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PABP (Methyl Arg455/460) Antibody, RBITC Conjugated (bs-3836R-RBITC)?
KLH conjugated synthetic metylpeptide derived from human PABP around metylation site of Arg455/Arg460
What are the excitation and emission wavelengths of the RBITC conjugate (bs-3836R-RBITC)?
RBITC on bs-3836R-RBITC: excitation / emission 540nm/625nm.