TSC1 Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3837R-PE-Cy5-100uL |
| Product Name | TSC1 Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Hamartin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7248 |
| Swiss Prot | Q92574 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | LAM; TSC1; Tuberous sclerosis 1 protein; TSC1_HUMAN. |
| Background | Hamartin, or TSC1, is a suspected tumor suppressor implicated in the disease tuberous sclerosis 1. It is a negative regulator of cell division controlling the transition from G0/G1 to S phase, and it seems to act through the phosphatidylinositol 3 kinase/Akt pathway. TSC1 interacts with tuberin m(TSC2), which is thought to be a GAP (GTPase Activating Protein) for the Rap1 and Rab5 small G Proteins. The Hamartin/Tuberin complex has been shown to inhibit mTor. Hamartin has also been shown to interact with ERM (Ezrin-Radixin-Moesin) proteins and with F-actin, suggesting a role for TSC proteins in modulation of cell adhesion and morphology. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is TSC1 Polyclonal Antibody, PE-Cy5 Conjugated (bs-3837R-PE-Cy5) validated for, and at what dilution?
bs-3837R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TSC1 Polyclonal Antibody, PE-Cy5 Conjugated (bs-3837R-PE-Cy5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse.
How should bs-3837R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TSC1 Polyclonal Antibody, PE-Cy5 Conjugated (bs-3837R-PE-Cy5)?
KLH conjugated synthetic peptide derived from human Hamartin
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-3837R-PE-Cy5)?
PE-Cy5 on bs-3837R-PE-Cy5: excitation / emission 488nm/670nm.